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Pre-packed columns, cartridges, and guard columns for separation of biomolecules and organic/inorganic analytes; includes normal/reversed phase HPLC, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.
The Discovery™ HS F5 bonded phase provides reversed-phase separations that are distinctly different from C18 columns. However, compounds will generally elute within the same retention time window, making most C18 methods easily transferable.
SUPELCOSIL™ LC-DB phases are specially deactivated for basic compounds. These columns provide less retention, better peak shape, and higher efficiency for organic bases than can be obtained on conventional reversed-phase columns.
A typical mobile phase used with normal-phase silica columns consists of a hydrocarbon solvent such as hexane or heptane, mixed with a relatively small percentage of more polar solvent.